Overview

  • Product nameAnti-SRC3 antibodySee all SRC3 primary antibodies ...
  • Description
    Rabbit polyclonal to SRC3
  • Tested applicationsIP, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 13 (LocusLink ID 8202).

  • General notes


    Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab10310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP
WB
  • Application notesIP: Use at a concentration of 20 µg/10mg extract.
    WB: 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
    • Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
    • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.
    • DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
    • Post-translational
      modifications
      Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
      Methylated by CARM1.
      Phosphorylated by IKK complex. Regulated its function.
    • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
    • Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ACTR antibody
      • ACTR antibody
      • AIB 1 antibody
      • AIB-1 antibody
      • AIB1 antibody
      • Amplified in breast cancer 1 antibody
      • Amplified in breast cancer 1 protein antibody
      • bHLHe42 antibody
      • CAGH 16 antibody
      • CAGH16 antibody
      • CBP interacting protein antibody
      • CBP-interacting protein antibody
      • Class E basic helix-loop-helix protein 42 antibody
      • CTG 26 antibody
      • CTG26 antibody
      • KAT13B / AIB1 antibody
      • MGC141848 antibody
      • NCOA 3 antibody
      • NCOA 3 antibody
      • NCoA-3 antibody
      • NCOA3 antibody
      • NCOA3_HUMAN antibody
      • Nuclear receptor coactivator 3 antibody
      • pCIP antibody
      • RAC 3 antibody
      • RAC-3 antibody
      • RAC3 antibody
      • Receptor associated coactivator 3 antibody
      • Receptor-associated coactivator 3 antibody
      • SRC 3 antibody
      • SRC-3 antibody
      • SRC3 antibody
      • Steroid receptor coactivator protein 3 antibody
      • Thyroid hormone receptor activator molecule 1 antibody
      • TNRC14 antibody
      • TNRC16 antibody
      • TRAM 1 antibody
      • TRAM-1 antibody
      • TRAM1 antibody
      see all

    Anti-SRC3 antibody images

    • Detection of SCR3 by Immunoprecipitation.

       Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.



    • Predicted band size : 170 kDa


      Detection of SCR3 by Immunoprecipitation. Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.

    References for Anti-SRC3 antibody (ab10310)

    This product has been referenced in:
    • Tilli MT  et al. Overexpression of an N-terminally truncated isoform of the nuclear receptor coactivator amplified in breast cancer 1 leads to altered proliferation of mammary epithelial cells in transgenic mice. Mol Endocrinol 19:644-56 (2005). WB ; Mouse . Read more (PubMed: 15550471) »

    See 1 Publication for this product

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