Anti-SRC3 antibody [AX 15] (ab12074)

Overview

  • Product nameAnti-SRC3 antibody [AX 15]See all SRC3 primary antibodies ...
  • Description
    Mouse monoclonal [AX 15] to SRC3
  • Tested applicationsIP, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein containing amino acids 605-1294 of human NCOA3.

  • Positive control
    • BG-1 ovarian cell RIPA lysate.
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Applications

Our Abpromise guarantee covers the use of ab12074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP
WB
  • Application notesIP: 15µl of ab12074 will immunoprecipitate NCOA3 from 0.5-1 mg of a BG-1 ovarian cell RIPA lysate.
    WB: 1/500. Ab12074 recognizes NCOA3 (150 kD) and another doublet (110 kD) by Western blotting a BG-1 ovarian cell RIPA lysate.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
    • Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
    • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.
    • DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
    • Post-translational
      modifications
      Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
      Methylated by CARM1.
      Phosphorylated by IKK complex. Regulated its function.
    • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
    • Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ACTR antibody
      • AIB 1 antibody
      • AIB-1 antibody
      • AIB1 antibody
      • Amplified in breast cancer 1 antibody
      • Amplified in breast cancer 1 protein antibody
      • bHLHe42 antibody
      • CAGH 16 antibody
      • CAGH16 antibody
      • CBP interacting protein antibody
      • CBP-interacting protein antibody
      • Class E basic helix-loop-helix protein 42 antibody
      • CTG 26 antibody
      • CTG26 antibody
      • KAT13B / AIB1 antibody
      • MGC141848 antibody
      • NCOA 3 antibody
      • NCOA 3 antibody
      • NCoA-3 antibody
      • NCOA3 antibody
      • NCOA3_HUMAN antibody
      • Nuclear receptor coactivator 3 antibody
      • Nuclear receptor coactivator 3 antibody
      • pCIP antibody
      • pCIP antibody
      • RAC 3 antibody
      • RAC-3 antibody
      • RAC3 antibody
      • Receptor associated coactivator 3 antibody
      • Receptor-associated coactivator 3 antibody
      • SRC 3 antibody
      • SRC-3 antibody
      • SRC3 antibody
      • Steroid receptor coactivator protein 3 antibody
      • Steroid receptor coactivator protein 3 antibody
      • Thyroid hormone receptor activator molecule 1 antibody
      • Thyroid hormone receptor activator molecule 1 antibody
      • TNRC14 antibody
      • TNRC16 antibody
      • TRAM 1 antibody
      • TRAM-1 antibody
      • TRAM1 antibody
      see all

    References for Anti-SRC3 antibody [AX 15] (ab12074)

    ab12074 has not yet been referenced specifically in any publications.

    Product Wall

    No we do not sell this lysate. You could try looking in Sigma's catalogue or perhaps searching for other providers on Biocompare.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"