Overview

  • Product name
    Anti-SRC3 antibody [AX15.3] - ChIP Grade
    See all SRC3 primary antibodies
  • Description
    Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade
  • Tested applications
    Suitable for: ChIP, WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human SRC3 aa 605-1294.

  • General notes
    Locus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.

Applications

Our Abpromise guarantee covers the use of ab2782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
ELISA Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.
IP Use at an assay dependent concentration.

Target

  • Function
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • Tissue specificity
    Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • Sequence similarities
    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain
    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • Post-translational
    modifications
    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACTR antibody
    • AIB 1 antibody
    • AIB-1 antibody
    • AIB1 antibody
    • Amplified in breast cancer 1 antibody
    • Amplified in breast cancer 1 protein antibody
    • bHLHe42 antibody
    • CAGH 16 antibody
    • CAGH16 antibody
    • CBP interacting protein antibody
    • CBP-interacting protein antibody
    • Class E basic helix-loop-helix protein 42 antibody
    • CTG 26 antibody
    • CTG26 antibody
    • KAT13B / AIB1 antibody
    • MGC141848 antibody
    • NCOA 3 antibody
    • NCoA-3 antibody
    • Ncoa3 antibody
    • NCOA3_HUMAN antibody
    • Nuclear receptor coactivator 3 antibody
    • pCIP antibody
    • RAC 3 antibody
    • RAC-3 antibody
    • RAC3 antibody
    • Receptor associated coactivator 3 antibody
    • Receptor-associated coactivator 3 antibody
    • SRC 3 antibody
    • SRC-3 antibody
    • SRC3 antibody
    • Steroid receptor coactivator protein 3 antibody
    • Thyroid hormone receptor activator molecule 1 antibody
    • TNRC14 antibody
    • TNRC16 antibody
    • TRAM 1 antibody
    • TRAM-1 antibody
    • TRAM1 antibody
    see all

Anti-SRC3 antibody [AX15.3] - ChIP Grade images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting



  • Predicted band size : 155 kDa
    Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.

References for Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

This product has been referenced in:
  • Wagner M  et al. NCOA3 is a selective co-activator of estrogen receptor a-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer 13:570 (2013). ChIP ; Human . Read more (PubMed: 24304549) »
  • Levy N  et al. Differential regulation of native estrogen receptor-regulatory elements by estradiol, tamoxifen, and raloxifene. Mol Endocrinol 22:287-303 (2008). ChIP ; Human . Read more (PubMed: 17962382) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7)
Loading amount
20000 cells
Specification
huh-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jun 16 2009

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