Overview

  • Product nameAnti-SRC3 antibody - ChIP GradeSee all SRC3 primary antibodies ...
  • Description
    Rabbit polyclonal to SRC3 - ChIP Grade
  • SpecificityDetects amplified in breast cancer 1 (AIB1).
  • Tested applicationsWB, IHC-P, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human SRC3 aa 3-15.
    Sequence:

    GLGENLDPLASDS


    (Peptide available as ab4915)

  • Positive control
    • T47D cell lysate

Applications

Our Abpromise guarantee covers the use of ab2831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 155 kDa.Can be blocked with SRC3 peptide (ab4915). Can be blocked with AIB1 peptide or reuse.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ChIP Use 3µg for 106 cells. PubMed: 18499756

Target

  • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • Tissue specificityWidely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • DomainContains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • Post-translational
    modifications
    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ACTR antibody
    • AIB 1 antibody
    • AIB-1 antibody
    • AIB1 antibody
    • Amplified in breast cancer 1 antibody
    • Amplified in breast cancer 1 protein antibody
    • bHLHe42 antibody
    • CAGH 16 antibody
    • CAGH16 antibody
    • CBP interacting protein antibody
    • CBP-interacting protein antibody
    • Class E basic helix-loop-helix protein 42 antibody
    • CTG 26 antibody
    • CTG26 antibody
    • KAT13B / AIB1 antibody
    • MGC141848 antibody
    • NCOA 3 antibody
    • NCoA-3 antibody
    • NCOA3 antibody
    • NCOA3_HUMAN antibody
    • Nuclear receptor coactivator 3 antibody
    • Nuclear receptor coactivator 3 antibody
    • pCIP antibody
    • RAC 3 antibody
    • RAC-3 antibody
    • RAC3 antibody
    • Receptor associated coactivator 3 antibody
    • Receptor-associated coactivator 3 antibody
    • SRC 3 antibody
    • SRC-3 antibody
    • SRC3 antibody
    • Steroid receptor coactivator protein 3 antibody
    • Steroid receptor coactivator protein 3 antibody
    • Thyroid hormone receptor activator molecule 1 antibody
    • Thyroid hormone receptor activator molecule 1 antibody
    • TNRC14 antibody
    • TNRC16 antibody
    • TRAM 1 antibody
    • TRAM-1 antibody
    • TRAM1 antibody
    see all

Anti-SRC3 antibody - ChIP Grade images



  • Predicted band size : 155 kDa
    Western blot of Human 2831 in T47D cell lysate using ab2831.
  • ab2831 can be used in ChIP. You can expect recruitment of AIB1on the pS2 promoter at some point during transcriptional activation. The amount of DNA precipiated is significant.

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab2831 to AIB1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab2831 and 2x106 cells were used in each ChIP experiment. 

  • ICC/IF image of ab2831 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2831, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • IHC image of ab2831 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2831, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SRC3 antibody - ChIP Grade (ab2831)

This product has been referenced in:
  • Mason CE  et al. Location analysis for the estrogen receptor-{alpha} reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res : (2010). ChIP ; Human . Read more (PubMed: 20047966) »
  • Fenne IS  et al. Recruitment of Coactivator GRIP1 to an Estrogen Receptor Transcription Complex is Regulated by the cAMP-dependent Protein Kinase. Endocrinology : (2008). ChIP ; Human . Read more (PubMed: 18499756) »

See all 3 Publications for this product

Product Wall

Thank you for the protocol details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab2831. The antibody was characterized for application in Western blotting using T47D cell lysate and a band at 155 kDa was detect...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"