Overview

  • Product nameAnti-SRD5A1 antibody
    See all SRD5A1 primary antibodies
  • Description
    Goat polyclonal to SRD5A1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SRD5A1 aa 173-184 (internal sequence).
    Sequence:

    RNLRKPGDTGYK


    Database link: P18405

  • Positive control
    • Human small intestine, liver and prostate tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab110123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 3.75 µg/ml. ab110123 was validated for use in Immunohistochemistry on a panel of 21 formalin fixed, paraffin embedded Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with a biotinylated Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. PubMed: 23405234

Target

  • FunctionConverts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • Tissue specificityLiver and prostate (at a low level).
  • Sequence similaritiesBelongs to the steroid 5-alpha reductase family.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 oxo 5 alpha steroid 4 dehydrogenase 1 antibody
    • 3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1 antibody
    • 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1,5-alpha reductase antibody
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 1 antibody
    • 5 alpha reductase antibody
    • S5A1_HUMAN antibody
    • S5AR 1 antibody
    • S5AR antibody
    • SR type 1 antibody
    • SRD5A 1 antibody
    • Srd5a1 antibody
    • Steroid 5 alpha reductase 1 antibody
    • Steroid 5 alpha reductase alpha polypeptide 1 (3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1) antibody
    • Steroid 5 alpha reductase alpha polypeptide 1 antibody
    • Steroid 5 alpha reductase type I antibody
    • Steroid 5-alpha-reductase 1 antibody
    see all

Anti-SRD5A1 antibody images

  • Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human small intestine, using ab110123 at 3.75 µg/ml.
  • Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human liver, using ab110123 at 3.75 µg/ml.
  • Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human prostate, using ab110123 at 3.75 µg/ml.

References for Anti-SRD5A1 antibody (ab110123)

This product has been referenced in:
  • Sánchez P  et al. Effects of different ethanol-administration regimes on mRNA and protein levels of steroid 5a-reductase isozymes in prefrontal cortex of adolescent male rats. Psychopharmacology (Berl) 231:3273-80 (2014). Rat . Read more (PubMed: 24714925) »
  • Castro B  et al. Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate. PLoS One 8:e55905 (2013). WB ; Rat . Read more (PubMed: 23405234) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate LNCaP cells)
Specification Prostate LNCaP cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Aug 17 2012

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