Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA 1/64000.

Target

  • Function
    Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • Tissue specificity
    Liver and prostate (at a low level).
  • Sequence similarities
    Belongs to the steroid 5-alpha reductase family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 oxo 5 alpha steroid 4 dehydrogenase 1 antibody
    • 3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1 antibody
    • 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1,5-alpha reductase antibody
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 1 antibody
    • 5 alpha reductase antibody
    • S5A1_HUMAN antibody
    • S5AR 1 antibody
    • S5AR antibody
    • SR type 1 antibody
    • SRD5A 1 antibody
    • Srd5a1 antibody
    • Steroid 5 alpha reductase 1 antibody
    • Steroid 5 alpha reductase alpha polypeptide 1 (3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1) antibody
    • Steroid 5 alpha reductase alpha polypeptide 1 antibody
    • Steroid 5 alpha reductase type I antibody
    • Steroid 5-alpha-reductase 1 antibody
    see all

Images

  • ab31295 at 4-6 µg/ml, staining SRD5A1 in Human Prostate tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Image shows reticulate staining in the cytoplasm of secretory cells in the gland. Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.

References

ab31295 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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