The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).Can be blocked with SRD5A2 peptide (2-13) (ab45681).
Use at an assay dependent concentration.
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Expressed in high levels in the prostate and many other androgen-sensitive tissues.
Involvement in disease
Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
Kelleher MA et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci20:1365-75 (2013).
Read more (PubMed: 23585339) »
Kelleher MA et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol208:301-9 (2011).
Read more (PubMed: 21149437) »