The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
1/10 - 1/100.
Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
FunctionConverts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Tissue specificityExpressed in high levels in the prostate and many other androgen-sensitive tissues.
Involvement in diseaseDefects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
Sequence similaritiesBelongs to the steroid 5-alpha reductase family.