Recombinant
RabMAb

Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)

Overview

  • Product name
    Anti-SRD5A2 antibody [EPR6281(B)]
    See all SRD5A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6281(B)] to SRD5A2
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of Human SRD5A2 (P31213).

  • Positive control
    • LnCaP, Human testis, and PC-3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
    • Tissue specificity
      Expressed in high levels in the prostate and many other androgen-sensitive tissues.
    • Involvement in disease
      Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
    • Sequence similarities
      Belongs to the steroid 5-alpha reductase family.
    • Cellular localization
      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
      • 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
      • 5 alpha SR2 antibody
      • 5 alpha-SR2 antibody
      • 5ART2 antibody
      • e II 5 alpha reductase antibody
      • EC antibody
      • MGC138457 antibody
      • Micropenis, included antibody
      • S5A2_HUMAN antibody
      • S5AR 2 antibody
      • SR type 2 antibody
      • SRD5A2 antibody
      • Steroid 5 alpha reductase 2 antibody
      • Steroid 5-alpha-reductase 2 antibody
      • Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) antibody
      • Type II 5-alpha reductase antibody
      see all

    Images

    • All lanes : Anti-SRD5A2 antibody [EPR6281(B)] (ab124877) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : Human testis lysate
      Lane 3 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124877 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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