Overview

  • Product name
    Anti-SRD5A3 antibody
  • Description
    Rabbit polyclonal to SRD5A3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide designed between amino acids 288-318 rom the C-terminal region of Human SRD5A3.

  • Positive control
    • K562 cell line lysates. Human prostate carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Probable polyprenol reductase. Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Probably acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
  • Cellular localization
    Cytoplasmic. Integral to endoplasmic reticulum membrane.
  • Database links
  • Alternative names
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 3 antibody
    • CDG1P antibody
    • probable polyprenol reductase antibody
    • S5AR 3 antibody
    • SR type 3 antibody
    • SRD5A2L antibody
    • SRD5A2L1 antibody
    • steroid 5 alpha reductase 2 like antibody
    • steroid 5 alpha-reductase 3 antibody
    see all

Images

  • Anti-SRD5A3 antibody (ab104063) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 37 kDa
  • IHC analysis of SRD5A3 expression in formalin fixed and paraffin embedded Human prostate carcinoma tissue using 1/50 ab104063 followed by a peroxidase conjugated secondary antibody and DAB staining.

References

ab104063 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab104063.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up