Overview

  • Product nameAnti-SREBP1 antibody
    See all SREBP1 primary antibodies
  • Description
    Rabbit polyclonal to SREBP1
  • Specificityab28481 recognises SREBP 1. B
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    MLQLINNQDSDFPGLF

    , corresponding to amino acids 32-47 of Mouse SREBP 1

  • Positive control
    • Mouse and rat liver. Rat kidney.

Applications

Our Abpromise guarantee covers the use of ab28481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Can be blocked with Mouse SREBP1 peptide (ab31099). Detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominant band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/2000.

Target

  • FunctionTranscriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
  • Tissue specificityExpressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
  • Sequence similaritiesBelongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
    Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
  • Cellular localizationNucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADD 1 antibody
    • bHLHd1 antibody
    • Class D basic helix-loop-helix protein 1 antibody
    • D630008H06 antibody
    • Processed sterol regulatory element-binding protein 1 antibody
    • SRBP1_HUMAN antibody
    • SREBF 1 antibody
    • SREBF1 antibody
    • SREBP 1 antibody
    • SREBP 1c antibody
    • SREBP-1 antibody
    • SREBP1 antibody
    • Sterol regulatory element binding protein 1 antibody
    • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
    • Sterol regulatory element binding transcription factor 1 antibody
    • Sterol regulatory element-binding transcription factor 1 antibody
    see all

Anti-SREBP1 antibody images

  • Immunocytochemical analysis of formalin-fixed NIH 3T3 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification is 60X
    The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.

  • Immunocytochemical analysis of formalin-fixed HepG2 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification was 60X
    The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.

  • Immunocytochemical analysis of formalin-fixed C2C12 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification was 60X
    The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.

  • All lanes : Anti-SREBP1 antibody (ab28481) at 1/1000 dilution

    Lane 1 : Mouse Liver Whole Cell Lysate
    Lane 2 : Rat Liver Whole Cell Lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-Conjugate

    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab28481 stained HepG2 cells. The cells were 10% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, goat anti rabbit DyLight® 488 IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab28481 staining SREBP1 in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 15 minutes at 25°C. Samples were incubated with primary antibody (1/2000 in goat serum) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-SREBP1 antibody (ab28481)

This product has been referenced in:
  • Jiao J  et al. Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet. Food Nutr Res 60:31304 (2016). WB ; Mouse . Read more (PubMed: 27616737) »
  • Segatto M  et al. Cholesterol metabolism is altered in Rett syndrome: a study on plasma and primary cultured fibroblasts derived from patients. PLoS One 9:e104834 (2014). WB ; Human . Read more (PubMed: 25118178) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat liver tissue lysate)
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Loading amount 25 µg
Specification Rat liver tissue lysate
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 23 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Permeabilization No
Fixative Formaldehyde
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Submitted Sep 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (rat liver tissue lysate)
Loading amount 140 µg
Specification rat liver tissue lysate
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Acetone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Submitted Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Acetone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Submitted Nov 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (liver)
Specification liver
Fixative Acetone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Sep 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Specification HepG2
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Sep 12 2012

Thank you for your reply.
The immunogen for ab28481 is conserved in all isoforms of SREBP1, and it should detect SREBP1.C. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us.

I am assuming customer is interested in molecular weight of proteins these antibodies detect. The predicted band size is as follows;

ab28481; the molecular weight of bands would be 121, 113, 111 and 124 kD...

Read More
Application Western blot
Sample Mouse Cell lysate - whole cell (Liver)
Loading amount 30 µg
Specification Liver
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3
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Submitted Jun 18 2007

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"