• Product name
  • Description
    Rabbit polyclonal to SREBP1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the Center region of Human SREBF1 (NP_004167.3).

  • Positive control
    • MDAMB435 cell line lysates



Our Abpromise guarantee covers the use of ab93333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 122 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
    • Tissue specificity
      Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
    • Sequence similarities
      Belongs to the SREBP family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
      Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
    • Cellular localization
      Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADD 1 antibody
      • bHLHd1 antibody
      • Class D basic helix-loop-helix protein 1 antibody
      • D630008H06 antibody
      • Processed sterol regulatory element-binding protein 1 antibody
      • SRBP1_HUMAN antibody
      • SREBF 1 antibody
      • SREBF1 antibody
      • SREBP 1 antibody
      • SREBP 1c antibody
      • SREBP-1 antibody
      • SREBP1 antibody
      • Sterol regulatory element binding protein 1 antibody
      • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
      • Sterol regulatory element binding transcription factor 1 antibody
      • Sterol regulatory element-binding transcription factor 1 antibody
      see all

    Anti-SREBP1 antibody images

    • Anti-SREBP1 antibody (ab93333) at 1/100 dilution + MDAMB435 cell line lysates at 35 µg

      Predicted band size : 122 kDa

    References for Anti-SREBP1 antibody (ab93333)

    ab93333 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab93333.
    Please use the links above to contact us or submit feedback about this product.


    Sign up