Overview

  • Product name
  • Description
    Rabbit polyclonal to SREBP1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the Center region of Human SREBF1 (NP_004167.3).

  • Positive control
    • MDAMB435 cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab93333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 122 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
    • Tissue specificity
      Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
    • Sequence similarities
      Belongs to the SREBP family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications
      At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
      Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
    • Cellular localization
      Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADD 1 antibody
      • bHLHd1 antibody
      • Class D basic helix-loop-helix protein 1 antibody
      • D630008H06 antibody
      • Processed sterol regulatory element-binding protein 1 antibody
      • SRBP1_HUMAN antibody
      • SREBF 1 antibody
      • SREBF1 antibody
      • SREBP 1 antibody
      • SREBP 1c antibody
      • SREBP-1 antibody
      • SREBP1 antibody
      • Sterol regulatory element binding protein 1 antibody
      • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
      • Sterol regulatory element binding transcription factor 1 antibody
      • Sterol regulatory element-binding transcription factor 1 antibody
      see all

    Images

    • Anti-SREBP1 antibody (ab93333) at 1/100 dilution + MDAMB435 cell line lysates at 35 µg

      Predicted band size : 122 kDa

    References

    ab93333 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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