Overview

  • Product name
  • Description
    Rabbit polyclonal to SREBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SREBP1 (internal sequence).
    Database link: NM_004176

  • Positive control
    • Human liver tissue

Applications

Our Abpromise guarantee covers the use of ab93638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • Function
    Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
  • Tissue specificity
    Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
  • Sequence similarities
    Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
    Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
  • Cellular localization
    Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADD 1 antibody
    • bHLHd1 antibody
    • Class D basic helix-loop-helix protein 1 antibody
    • D630008H06 antibody
    • Processed sterol regulatory element-binding protein 1 antibody
    • SRBP1_HUMAN antibody
    • SREBF 1 antibody
    • SREBF1 antibody
    • SREBP 1 antibody
    • SREBP 1c antibody
    • SREBP-1 antibody
    • SREBP1 antibody
    • Sterol regulatory element binding protein 1 antibody
    • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
    • Sterol regulatory element binding transcription factor 1 antibody
    • Sterol regulatory element-binding transcription factor 1 antibody
    see all

Images

  • ab93638 at 1/100 dilution, showing membrane staining of SREBP1 in formalin-fixed, paraffin-embedded Human liver tissue by Immunohistochemistry.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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