Anti-SREBP2 antibody (ab194667)
Key features and details
- Mouse polyclonal to SREBP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SREBP2 antibody
See all SREBP2 primary antibodies -
Description
Mouse polyclonal to SREBP2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human SREBP2 aa 800-900. (AAH56158).
Database link: Q12772 -
Positive control
- IMR-32 cell lysate; recombinant Human SREBP2 protein (immunogen).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 123 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 123 kDa. |
Target
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Function
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. -
Tissue specificity
Ubiquitously expressed in adult and fetal tissues. -
Sequence similarities
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAt low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. -
Cellular localization
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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Database links
- Entrez Gene: 100689017 Chinese hamster
- Entrez Gene: 6721 Human
- Entrez Gene: 20788 Mouse
- Entrez Gene: 300095 Rat
- Omim: 600481 Human
- SwissProt: Q60429 Chinese hamster
- SwissProt: Q12772 Human
- SwissProt: Q3U1N2 Mouse
see all -
Alternative names
- AI608257 antibody
- bHLHd2 antibody
- Class D basic helix-loop-helix protein 2 antibody
see all
Images
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Anti-SREBP2 antibody (ab194667) at 1/500 dilution + IMR-32 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 123 kDa -
Anti-SREBP2 antibody (ab194667) at 1/1000 dilution + Recombinant Human SREBP2 protein (immunogen) at 0.2 µg
Developed using the ECL technique.
Predicted band size: 123 kDaPredicted MWt of immunogen: 37.0 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab194667 has been referenced in 1 publication.
- Lee W et al. COVID-19-activated SREBP2 disturbs cholesterol biosynthesis and leads to cytokine storm. Signal Transduct Target Ther 5:186 (2020). PubMed: 32883951