Overview

  • Product nameAnti-SREBP2 antibody
    See all SREBP2 primary antibodies
  • Description
    Rabbit polyclonal to SREBP2
  • Specificityab28482 recognises Sterol regulatory element-binding protein 2 (SREBP 2)
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Guinea pig, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Rat SREBP2 aa 843-857.
    Sequence:

    AISWLQGDDAAVRSH

  • Positive control
    • Mouse and rat liver. Rat kidney.

Applications

Our Abpromise guarantee covers the use of ab28482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 68, 120 kDa (predicted molecular weight: 120 kDa).
ICC/IF 1/20 - 1/200.
IHC-P Use a concentration of 2 µg/ml.

Target

  • FunctionTranscriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
  • Tissue specificityUbiquitously expressed in adult and fetal tissues.
  • Sequence similaritiesBelongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
  • Cellular localizationNucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI608257 antibody
    • bHLHd2 antibody
    • Class D basic helix-loop-helix protein 2 antibody
    • OTTHUMP00000028740 antibody
    • Processed sterol regulatory element-binding protein 2 antibody
    • SRBP2_HUMAN antibody
    • SREBF 2 antibody
    • Srebf2 antibody
    • SREBF2 protein antibody
    • SREBP 2 antibody
    • SREBP-2 antibody
    • SREBP2 antibody
    • SREBP2gc antibody
    • sterol regulatory element binding factor 2 antibody
    • Sterol regulatory element binding protein 2 antibody
    • Sterol regulatory element binding transcription factor 2 antibody
    • Sterol regulatory element-binding transcription factor 2 antibody
    see all

Anti-SREBP2 antibody images

  • Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of L6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ab28482 (2µg/ml) staining SREPB 2 in human temporal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the microvasculature, white matter, granule neutrophil and glial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-SREBP2 antibody (ab28482)

This product has been referenced in:

See all 7 Publications for this product

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DISCOUNT CODE: 100%ABR-XXXXXX
Expiration date: 26/05/2012

I am very pleased to hear you would like to accept our offer and test ab28482 in Bovine. This code will give you 1 free P...

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Thank you very much for your interest in ab28482.

To our knowledge,ab28482 has not been tested in bovine. Therefore, I can offer a discount off a future purchase if you buyab28482 now, test it inin bovine and submit feedback to us in the for...

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Thank you for contacting us. Indeed, I can confirm that the storage buffer of anti-SREBP2 antibody ab28482 (lot859967) is PBS containing 0.05% sodium azide and 1 mg/ml BSA as a stabilizing protein. This is needed to reduce the risk of degradation b...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela whole cell lysates)
Loading amount 1 µg
Specification Hela whole cell lysates
Gel Running Conditions Reduced Denaturing (4-20%, 15well, 1.5mm)
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 37°C
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Verified customer

Submitted Oct 20 2010

Thank you for your enquiry. Regarding ab28482 (SREBP 2), this product does detects an ~68 and 120 kDa protein representing SREBP 2 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site)...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"