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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-SREBP2 antibody
See all SREBP2 products (4) ...
Description
Rabbit polyclonal to SREBP2
Specificity
Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. ab30682 detects both precursor and active forms of SREBP2 in tissues and cells such as liver, brown fat, testis, HepG2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.
Tested applications
IHC-Fr, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Predicted to work with
Pig, Xenopus laevis, Chinese Hamster
Immunogen
Synthetic peptide: SPLLDDAKVKDEPDS, corresponding to amino acids 455 - 469 of Human SREBP2
SPLLDDAKVK DEPDS
Positive control
Rat testis supernatant.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/100IHC-Fr: 1/100
WB: Use a concentration of 4 µg/mlDetects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).(Block with 0.5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4 °C.)
ICC: Use a concentration of 4 µg/ml
Target
Function
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
Tissue specificity
Ubiquitously expressed in adult and fetal tissues.
Sequence similarities
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Cellular localization
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Target information above from: UniProt accessionQ12772
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI608257 antibody
- bHLHd2 antibody
- Class D basic helix-loop-helix protein 2 antibody
see all
Database links
- Entrez Gene: 395304 Chicken
- Entrez Gene: 6721 Human
- Entrez Gene: 20788 Mouse
- Entrez Gene: 396675 Pig
- Entrez Gene: 300095 Rat
- Entrez Gene: 443980 Xenopus laevis
- Omim: 600481 Human
- SwissProt: Q60429 Chinese Hamster
see all
Anti-SREBP2 antibody images:
Western blot - SREBP2 antibody (ab30682)
All lanes : Anti-SREBP2 antibody (ab30682) at 2.5 µg/ml
Lane 1 : Human fibroblast cell lysate
Lane 2 : Rat brown fat homogenate
Lane 3 : Rat testis supernatant
Lysates/proteins at 60 µg per lane.
Predicted band size : 126 kDa
Observed bands 126 kDa, 55kDa (cleaved form)
Immunocytochemistry/ Immunofluorescence - SREBP2 antibody (ab30682)
ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-SREBP2 antibody (ab30682)
This product has been referenced in:
- Johnson KJet al. Species-specific dibutyl phthalate fetal testis endocrine disruption correlates with inhibition of SREBP2-dependent gene expression pathways. Toxicol Sci 120:460-74 (2011). Rat.Read more (PubMed: 21266533) »
- Spencer CMet al. Human cytomegalovirus induces the activity and expression of acetyl-coenzyme A carboxylase, a fatty acid biosynthetic enzyme whose inhibition attenuates viral replication. J Virol 85:5814-24 (2011).Read more (PubMed: 21471234) »
See all 9 publications for this product
Publishing research using ab30682? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"