Recombinant fragment: PLDPETIAQD IEETMNTALN ELRELERQST AKHAPDVVLD TLEQVKNSPT PATSTESLSP LHNVALRSSE PQIRRSTSSS SDTMSTFKPM VAPRMGVQL, corresponding to amino acids 952-1051 of Human SRGAP1
Our Abpromise guarantee covers the use of ab57504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use a concentration of 3 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
ICC/IF image of ab57504 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab57504 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.