Overview

  • Product name
  • Description
    Rabbit polyclonal to SRGAP1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 825 and 875 of human SRGAP1 (NP_065813.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
    • Tissue specificity
      Expressed in brain, lung, kidney, and testis.
    • Sequence similarities
      Contains 1 FCH domain.
      Contains 1 Rho-GAP domain.
      Contains 1 SH3 domain.
    • Domain
      The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930572H05Rik antibody
      • ARHGAP13 antibody
      • FLJ22166 antibody
      • KIAA1304 antibody
      • RGD1566260 antibody
      • Rho GTPase activating protein 13 antibody
      • Rho GTPase-activating protein 13 antibody
      • SLIT ROBO Rho GTPase activating protein 1 antibody
      • SLIT-ROBO Rho GTPase-activating protein 1 antibody
      • SRGAP 1 antibody
      • srGAP1 antibody
      • SRGP1_HUMAN antibody
      see all

    Anti-SRGAP1 antibody images

    • Detection of SRGAP1 by Western Blot of Immunprecipitate.
      anti-SRGAP1 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab75281 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 1 second.

    References for Anti-SRGAP1 antibody (ab75281)

    ab75281 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Sample
    Mouse Tissue sections (mouse embryonic spinal cord E11)
    Specification
    mouse embryonic spinal cord E11
    Permeabilization
    Yes - 0,25%TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Alain Chedotal

    Verified customer

    Submitted May 17 2013

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
    Loading amount
    30 µg
    Specification
    HEK293H, COS7, mouse hindbrain lysate E14
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS gradient gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Alain Chedotal

    Verified customer

    Submitted Apr 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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