Anti-SRGAP1 antibody (ab76926)
Key features and details
- Rabbit polyclonal to SRGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SRGAP1 antibody
See all SRGAP1 primary antibodies -
Description
Rabbit polyclonal to SRGAP1 -
Host species
Rabbit -
Specificity
Does not crossreact with human srGAP2 or human srGAP3 -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residue 979 and 1029 of human SRGAP1 (NP_065813.1)
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab76926 was affinity purified using an epitope specific to SRGAP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42. -
Tissue specificity
Expressed in brain, lung, kidney, and testis. -
Sequence similarities
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. -
Domain
The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions. - Information by UniProt
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Database links
- Entrez Gene: 57522 Human
- Omim: 606523 Human
- SwissProt: Q7Z6B7 Human
- Unigene: 210751 Human
- Unigene: 593803 Human
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Alternative names
- 4930572H05Rik antibody
- ARHGAP13 antibody
- FLJ22166 antibody
see all
Images
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All lanes : Anti-SRGAP1 antibody (ab76926) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Lane 4 : 293T whole cell lysate. at 50 µg
Predicted band size: 124 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
ab76926 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab76926 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab76926 has been referenced in 2 publications.
- Chiang TS et al. ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation. Mol Biol Cell 30:69-81 (2019). PubMed: 30427759
- Genet G et al. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nat Commun 10:2350 (2019). PubMed: 31138815