Overview

  • Product nameAnti-SRGAP1 antibody
    See all SRGAP1 primary antibodies
  • Description
    Rabbit polyclonal to SRGAP1
  • SpecificityDoes not crossreact with human srGAP2 or human srGAP3
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 979 and 1029 of human SRGAP1 (NP_065813.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionGTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
  • Tissue specificityExpressed in brain, lung, kidney, and testis.
  • Sequence similaritiesContains 1 FCH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • DomainThe F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930572H05Rik antibody
    • ARHGAP13 antibody
    • FLJ22166 antibody
    • KIAA1304 antibody
    • RGD1566260 antibody
    • Rho GTPase activating protein 13 antibody
    • Rho GTPase-activating protein 13 antibody
    • SLIT ROBO Rho GTPase activating protein 1 antibody
    • SLIT-ROBO Rho GTPase-activating protein 1 antibody
    • SRGAP 1 antibody
    • srGAP1 antibody
    • SRGP1_HUMAN antibody
    see all

Anti-SRGAP1 antibody images

  • All lanes : Anti-SRGAP1 antibody (ab76926) at 1/2000 dilution

    Lane 1 : COS7 Whole cell lysate
    Lane 2 : HEK293H Whole cell lysate
    Lane 3 : HEK293H Whole cell lysate overexpressing human srGAP1
    Lane 4 : HEK293H whole cell lysate overexpressing human srGAP2
    Lane 5 : HEK293H whole cell lysate overexpressing human srGAP3
    Lane 6 : HeLa whole cell lysate overexpressing mouse srGAP2
    Lane 7 : Mouse E14 hindbrain lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 124 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 170 kDa (possible tagged protein).

    Exposure time : 5 minutes

    Image courtesy of Alain Chedotal (Institut Vision, France)

    Blocking: 5% Milk

  • All lanes : Anti-SRGAP1 antibody (ab76926) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate. at 50 µg
    Lane 2 : HeLa whole cell lysate. at 15 µg
    Lane 3 : HeLa whole cell lysate. at 5 µg
    Lane 4 : 293T whole cell lysate. at 50 µg


    Predicted band size : 124 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • ab76926 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab76926 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References for Anti-SRGAP1 antibody (ab76926)

ab76926 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step 0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0,25%TritonX100
Fixative Paraformaldehyde
Username

Dr. Alain Chedotal

Verified customer

Submitted May 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
Loading amount 30 µg
Specification HEK293H, COS7, mouse hindbrain lysate E14
Gel Running Conditions Reduced Denaturing (4-15% SDS gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Apr 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"