Recombinant Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8760(2)] to SRGAP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SRGAP1 antibody [EPR8760(2)]
See all SRGAP1 primary antibodies -
Description
Rabbit monoclonal [EPR8760(2)] to SRGAP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SRGAP1 aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q7Z6B7 -
Positive control
- Human fetal brain, Human fetal lung, Human fetal kidney tissue lysates, A549 cells, 293T cell line lysate, Human colon tissue, Human lung tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8760(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42. -
Tissue specificity
Expressed in brain, lung, kidney, and testis. -
Sequence similarities
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. -
Domain
The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions. - Information by UniProt
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Database links
- Entrez Gene: 57522 Human
- Entrez Gene: 117600 Mouse
- Entrez Gene: 314903 Rat
- Omim: 606523 Human
- SwissProt: Q7Z6B7 Human
- SwissProt: Q91Z69 Mouse
- Unigene: 210751 Human
- Unigene: 593803 Human
see all -
Alternative names
- 4930572H05Rik antibody
- ARHGAP13 antibody
- FLJ22166 antibody
see all
Images
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All lanes : Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : 293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Human fetal lung tissue lysate
Lane 5 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 124 kDa -
Intracellular flow cytometric analysis of permeabilized 293T cellslabeling SRGAP1 with ab171938 at 1/100 (red)or a rabbit IgG (negative) (green).
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Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).
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Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab171938 has not yet been referenced specifically in any publications.