Overview

  • Product nameAnti-srGAP2 antibody
    See all srGAP2 primary antibodies
  • Description
    Rabbit polyclonal to srGAP2
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: WEGRHNGIDG LIPHQYIVVQ DTEDGVVERS SPKSEIEVIS EPPEEKVTAR AGASCPSGGH VADIYLANIN KQRKRPESGS IR, corresponding to amino acids 766-847 of Human srGAP2 (O75044).

  • Positive control
    • Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • FunctionPutative GTPase-activating protein for Rho family small GTPases.
  • Tissue specificityExpression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested.
  • Sequence similaritiesContains 1 FCH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGAP34 antibody
    • FBP2 antibody
    • FNBP2 antibody
    • FNBP2_HUMAN antibody
    • Formin binding protein 2 antibody
    • Formin-binding protein 2 antibody
    • Rho GTPase-activating protein 34 antibody
    • SLIT ROBO Rho GTPase activating protein 2 antibody
    • SLIT-ROBO Rho GTPase-activating protein 2 antibody
    • srGAP2 antibody
    • SRGAP3 antibody
    see all

Anti-srGAP2 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121976 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121976 at 1/20 dilution staining srGAP2 in formalin-fixed, paraffin-embedded Human duodenum tissue by immunohistochemistry.

References for Anti-srGAP2 antibody (ab121976)

ab121976 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step 0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0,25%TritonX100
Fixative Paraformaldehyde
Username

Dr. Alain Chedotal

Verified customer

Submitted May 17 2013

Western blot

Below Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-15% SDS gradient gel)
Sample Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
Specification HEK293H, COS7, mouse hindbrain lysate E14
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Alain Chedotal

Verified customer

Submitted May 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"