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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer pH: 7.20 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Purity Immunogen affinity purified
Primary antibody notes ab121977 is mono-specific.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100. Detects a band of approximately 170 kDa (predicted molecular weight: 121 kDa).
Abcam recommends 5% milk blocking
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Function Putative GTPase-activating protein for Rho family small GTPases.
Tissue specificity Expression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested.
Sequence similarities Contains 1 FCH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.
Information by UniProt
Anti-srGAP2 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (ab121977)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-srGAP2 antibody (ab121977) This image is courtesy of Alain Chedotal (Institut Vision, France)
All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution Lane 1 : COS7 Whole cell lysate Lane 2 : HEK293H Whole Cell Lysate Lane 3 : HEK293H Over expressing human srGAP1 Lane 4 : HEK293H Over expressing human srGAP2 Lane 5 : HEK293H Over expressing human srGAP3 Lane 6 : HeLa cells over expressing mouse srGAP2 Lane 7 : Mouse E14 hindbrain lysate Lysates/proteins at 30 µg per lane. Secondary Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 121 kDa Observed band size : 170 kDa ( why is the actual band size different from the predicted?) Additional bands at : 200 kDa (possible tagged protein). Exposure time : 5 minutes This image is courtesy of Alain Chedotal (Institut Vision, France) Blocking: 5% MIlk
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (ab121977)
ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
References for Anti-srGAP2 antibody (ab121977)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"