• Product nameAnti-SRP1 antibody
    See all SRP1 primary antibodies
  • Description
    Mouse monoclonal to SRP1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-539 of Human SRP1



Our Abpromise guarantee covers the use of ab55387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 24625024


  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Post-translational
    Polyubiquitinated in the presence of RAG1 (in vitro).
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMA5_HUMAN antibody
    • Importin alpha 1 subunit antibody
    • Importin alpha 5 antibody
    • Importin alpha S1 antibody
    • Importin subunit alpha-5, N-terminally processed antibody
    • IPO A5 antibody
    • IPOA 5 antibody
    • IPOA5 antibody
    • Karyopherin alpha 1 antibody
    • Karyopherin alpha 1 subunit antibody
    • Karyopherin subunit alpha-1 antibody
    • KPNA 1 antibody
    • KPNA1 antibody
    • mSRP 1 antibody
    • mSRP1 antibody
    • NPI 1 antibody
    • NPI-1 antibody
    • NPI1 antibody
    • Nucleoprotein interactor 1 antibody
    • RAG cohort protein 2 antibody
    • RCH 2 antibody
    • RCH2 antibody
    • Recombination activating gene cohort 2 antibody
    • SRP 1 antibody
    • SRP1 beta antibody
    • SRP1-beta antibody
    see all

Anti-SRP1 antibody images

  • SRP1 antibody (ab55387) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human testis.

  • Predicted band size : 60 kDa
    SRP1 antibody (ab55387) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-SRP1 antibody (ab55387)

This product has been referenced in:
  • Zaza G  et al. Karyopherins: potential biological elements involved in the delayed graft function in renal transplant recipients. BMC Med Genomics 7:14 (2014). ICC/IF ; Human . Read more (PubMed: 24625024) »
  • Martrat G  et al. Exploring the link between MORF4L1 and risk of breast cancer. Breast Cancer Res 13:R40 (2011). Read more (PubMed: 21466675) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 40 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Jun 30 2011