Overview

  • Product nameAnti-SRP1 antibody
    See all SRP1 primary antibodies
  • Description
    Goat polyclonal to SRP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: TTPGKENFRLKSY, corresponding to N terminal amino acids 2-14 of Human SRP1.

  • Positive control
    • Hela and Human Brain lysates.
  • General notesGenBank Accession Number – NP_002255.


    Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The KPNA1 product interacts with RAG1 and may play a role in V(D)J recombination.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesRecombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The KPNA1 product interacts with RAG1 and may play a role in V(D)J recombination.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab6035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human SRP1 peptide (ab23141).

Target

  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of RAG1 (in vitro).
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMA5_HUMAN antibody
    • Importin alpha 1 subunit antibody
    • Importin alpha 5 antibody
    • Importin alpha S1 antibody
    • Importin subunit alpha-5, N-terminally processed antibody
    • IPO A5 antibody
    • IPOA 5 antibody
    • IPOA5 antibody
    • Karyopherin alpha 1 antibody
    • Karyopherin alpha 1 subunit antibody
    • Karyopherin subunit alpha-1 antibody
    • KPNA 1 antibody
    • KPNA1 antibody
    • mSRP 1 antibody
    • mSRP1 antibody
    • NPI 1 antibody
    • NPI-1 antibody
    • NPI1 antibody
    • Nucleoprotein interactor 1 antibody
    • RAG cohort protein 2 antibody
    • RCH 2 antibody
    • RCH2 antibody
    • Recombination activating gene cohort 2 antibody
    • SRP 1 antibody
    • SRP1 beta antibody
    • SRP1-beta antibody
    see all

Anti-SRP1 antibody images

  • Anti-SRP1 antibody (ab6035) at 1 µg/ml + HeLa lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-SRP1 antibody (ab6035)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"