Overview

  • Product nameAnti-SRP1 antibody
    See all SRP1 primary antibodies
  • Description
    Rabbit polyclonal to SRP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50, MTTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV of Human SRP1 (NP_002255).

  • Positive control
    • 721_B Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of RAG1 (in vitro).
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMA5_HUMAN antibody
    • Importin alpha 1 subunit antibody
    • Importin alpha 5 antibody
    • Importin alpha S1 antibody
    • Importin subunit alpha-5, N-terminally processed antibody
    • IPO A5 antibody
    • IPOA 5 antibody
    • IPOA5 antibody
    • Karyopherin alpha 1 antibody
    • Karyopherin alpha 1 subunit antibody
    • Karyopherin subunit alpha-1 antibody
    • KPNA 1 antibody
    • KPNA1 antibody
    • mSRP 1 antibody
    • mSRP1 antibody
    • NPI 1 antibody
    • NPI-1 antibody
    • NPI1 antibody
    • Nucleoprotein interactor 1 antibody
    • RAG cohort protein 2 antibody
    • RCH 2 antibody
    • RCH2 antibody
    • Recombination activating gene cohort 2 antibody
    • SRP 1 antibody
    • SRP1 beta antibody
    • SRP1-beta antibody
    see all

Anti-SRP1 antibody images



  • Predicted band size : 60 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling SRP1 with ab81448 at 1.0µg/ml.


  • Predicted band size : 60 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling SRP1 with ab81448 at 1.0µg/ml.


  • Predicted band size : 60 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling SRP1 with ab81448 at 1.0µg/ml.
  • Anti-SRP1 antibody (ab81448) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    Gel Concentration: 12%

References for Anti-SRP1 antibody (ab81448)

ab81448 has not yet been referenced specifically in any publications.

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