Overview

  • Product nameAnti-SRP19 antibody
    See all SRP19 primary antibodies
  • Description
    Rabbit polyclonal to SRP19
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: VQFPSRKSVM LYAAEMIPKL KTRTQKTGGA DQSLQQGEGS KKGKGKKKK, corresponding to amino acids 96-144 of Human SRP19

  • Positive control
    • Human skin, Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/1.5625e+006.

ELISA: Titre using peptide based assay 1/1562500.

Target

Anti-SRP19 antibody images

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-SRP19 antibody (ab50932) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • Ab50932 at 4.0 µg/ml staining squamous epithelial cells from Human skin; paraffin embedded, magnification 400x

References for Anti-SRP19 antibody (ab50932)

ab50932 has not yet been referenced specifically in any publications.

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I can cofirm that the immunogen sequence is from amino acid 96-144 of human SRP19.Itwas a full ˜48 aa peptide.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"