Overview

  • Product name
  • Description
    Rabbit polyclonal to SRP68
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Common marmoset
  • Immunogen

    Synthetic peptide, corresponding to a region within the N terminal amino acids 1-50 of Human SRP68 (NP_055045.2).

  • Positive control
    • HeLa, 293T, TCMK 1, Renca, NIH 3T3, and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab157120 was affinity purified using an epitope specific to SRP68 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
  • Sequence similarities
    Belongs to the SRP68 family.
  • Domain
    The RNA-binding domain is located near the N-terminus.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal recognition particle 68 antibody
    • Signal recognition particle 68 kDa antibody
    • Signal recognition particle 68 kDa protein antibody
    • signal recognition particle 68kD antibody
    • Signal recognition particle 68kDa antibody
    • signal recognition particle subunit SRP68 antibody
    • SRP 68 antibody
    • SRP68 antibody
    • SRP68_HUMAN antibody
    see all

Images

  • All lanes : Anti-SRP68 antibody (ab157120) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-SRP68 antibody (ab157120) at 0.1 µg/ml

    Lane 1 : TCMK 1 whole cell lysate
    Lane 2 : Renca whole cell lysate
    Lane 3 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 70 kDa


    Exposure time: 30 seconds
  • Detection of SRP68 by Western Blot of Immunprecipitate.
    ab157120 at 0.4µg/ml labeling SRP68 in HeLa whole cell lysate immunoprecipitated using ab157120 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab157120 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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