Overview

  • Product name
  • Description
    Rabbit polyclonal to SRPK1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SRPK1 aa 290-480.
    Sequence:

    E EMEKESGPGQ KRPNKQEESE SPVERPLKEN PPNKMTQEKL EESSTIGQDQ TLMERDTEGG AAEINCNGVI EVINYTQNSN NETLRHKEDL HNANDCDVQN LNQESSFLSS QNGDSSTSQE TDSCTPITSE VSDTMVCQSS STVGQSFSEQ HISQLQESIR AEIPCEDEQE QEHNGPLDNK GKSTAGNFLV NPLEPKNAEK


    Database link: Q96SB4

  • Positive control
    • 293T; MCF7; HeLa and THP1 cell extracts. Rat testis and mouse spleen extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function
    Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
  • Tissue specificity
    Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine/arginine rich protein specific kinase 1 antibody
    • Serine/arginine rich splicing factor kinase 1 antibody
    • Serine/arginine-rich protein-specific kinase 1 antibody
    • Serine/threonine protein kinase SRPK1 antibody
    • Serine/threonine-protein kinase SRPK1 antibody
    • SFRS protein kinase 1 antibody
    • SFRSK1 antibody
    • SR protein kinase 1 antibody
    • SR protein specific kinase 1 antibody
    • SR-protein-specific kinase 1 antibody
    • SRPK1 antibody
    • SRPK1_HUMAN antibody
    see all

Images

  • All lanes : Anti-SRPK1 antibody (ab189839) at 1/500 dilution

    Lane 1 : 293T cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : MCF7 cell extract
    Lane 4 : HeLa cell extract
    Lane 5 : THP1 cell extract
    Lane 6 : Rat testis extract
    Lane 7 : Mouse spleen extract


    Predicted band size : 74 kDa

References

ab189839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189839.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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