Overview

  • Product nameAnti-SRPK1 antibody
    See all SRPK1 primary antibodies
  • Description
    Rabbit polyclonal to SRPK1
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human SRPK1 aa 275-325 (internal sequence). The exact sequence is proprietary. (GeneID 6732)
    Database link: NP_003128.3

  • Positive control
    • Whole cell lysate from HeLa cells, 293T cells and NIH 3T3 cells. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionPlays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
  • Tissue specificityIsoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine/arginine rich protein specific kinase 1 antibody
    • Serine/arginine rich splicing factor kinase 1 antibody
    • Serine/arginine-rich protein-specific kinase 1 antibody
    • Serine/threonine protein kinase SRPK1 antibody
    • Serine/threonine-protein kinase SRPK1 antibody
    • SFRS protein kinase 1 antibody
    • SFRSK1 antibody
    • SR protein kinase 1 antibody
    • SR protein specific kinase 1 antibody
    • SR-protein-specific kinase 1 antibody
    • SRPK1 antibody
    • SRPK1_HUMAN antibody
    see all

Anti-SRPK1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling SRPK1 with ab90527 at 1/200 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab90527 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90527 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HepG2, Hek293, MCF-7 cells at 1ug/ml, and also in formaldehyde fixed (4%, 10min) HeLa, Hek293, HepG2, MCF-7 cells at 1ug/ml.

  • All lanes : Anti-SRPK1 antibody (ab90527) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 92 kDa


    Exposure time : 10 seconds
  • ab90527 at 1 µg/ml detecting SRPK1 following immunoprecipitation. Detection by ECL with a 3 second exposure.
    Lane 1; IP with ab90527
    Lane 2; IP with an antibody to a downstream epitope of SRPK1
    Lane 3; IP with control IgG
    All antibodies used at at 3 µg/mg lysate for immunoprecipitation. 1 mg of lysate was used for IP and 20% of IP loaded.

References for Anti-SRPK1 antibody (ab90527)

ab90527 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse muscle cells)
Loading amount 20 µg
Specification mouse muscle cells
Gel Running Conditions Reduced Denaturing (4-12% nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"