• Product name
  • Description
    Mouse polyclonal to SRPK2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length SRPK2 protein (Human)

  • Positive control
    • SRPK2 transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab67993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75, 140 kDa (predicted molecular weight: 78 kDa).


  • Function
    Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.
  • Tissue specificity
    Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.
    Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Human serine kinase SRPK2 mRNA, complete cds antibody
    • Serine kinase SRPK2 antibody
    • Serine/arginine rich protein specific kinase 2 antibody
    • Serine/arginine-rich protein-specific kinase 2 antibody
    • Serine/threonine protein kinase SRPK2 antibody
    • SFRS protein kinase 2 antibody
    • SFRSK2 antibody
    • SR protein specific kinase 2 antibody
    • SR-protein-specific kinase 2 antibody
    • SRPK2 antibody
    • SRPK2_HUMAN antibody
    • SRSF protein kinase 2 C-terminal antibody
    see all


  • All lanes : Anti-SRPK2 antibody (ab67993) at 1/500 dilution

    Lane 1 : SRPK2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 78 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:
  • Huang Y  et al. Global tumor protein p53/p63 interactome: Making a case for cisplatin chemoresistance. Cell Cycle 11:2367-79 (2012). WB ; Human . Read more (PubMed: 22672905) »

See 1 Publication for this product

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