Recombinant fragment, corresponding to amino acids 39-295 of Dog SRPR alpha, which includes part of the amino terminal SRPR beta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.
Canine microsomal protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at an assay dependent dilution. Can be used to immunoprecipitate both subunits of SRPR under non-denaturing conditions.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 72 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system.
Sequence similaritiesBelongs to the GTP-binding SRP family.
Cellular localizationEndoplasmic reticulum membrane. Thought to be anchored in the membrane through an interaction with SR-beta, which contains a bona fide transmembrane domain.