Overview

  • Product name
  • Description
    Rabbit polyclonal to SRRM2
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag antigen, corresponding to amino acids 1098 - 1203 of Human SRRM2 Isoforms 1 and 2 (Q9UQ35). Note: this sequence is missing in Isoform 3.

  • Positive control
    • Human liver tissue
      Human cell line U-2 OS

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Involved in pre-mRNA splicing. May function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome. May do so by stabilizing the catalytic center or the position of the RNA substrate (By similarity). Binds to RNA.
  • Tissue specificity
    Expressed in liver, placenta, and white blood cells.
  • Sequence similarities
    Belongs to the CWC21 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • 300 kDa nuclear matrix antigen antibody
    • CWC21 antibody
    • KIAA0324 antibody
    • Ser/Arg-related nuclear matrix protein of 300 kDa antibody
    • Serine/arginine repetitive matrix protein 2 antibody
    • Serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa antibody
    • Splicing coactivator subunit SRm300 antibody
    • SR-related nuclear matrix protein of 300 kDa antibody
    • SRL300 antibody
    • SRM300 antibody
    • SRRM2 antibody
    • SRRM2_HUMAN antibody
    • Tax-responsive enhancer element-binding protein 803 antibody
    • TaxREB803 antibody
    see all

Images

  • ab122719, at a 1/500 dilution, staining SRRM2 in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab122719, at 4 ug/ml, staining SRRM2 in Human cell line U-2 OS by Immunoflouroscence.

References

This product has been referenced in:
  • Bai B  et al. U1 small nuclear ribonucleoprotein complex and RNA splicing alterations in Alzheimer's disease. Proc Natl Acad Sci U S A 110:16562-7 (2013). IHC-FrFl ; Human . Read more (PubMed: 24023061) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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