Overview

  • Product name
    Anti-SRY antibody - N-terminal
    See all SRY primary antibodies
  • Description
    Rabbit polyclonal to SRY - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Mouse SRY aa 1-144 (N terminal).
    Sequence:

    MEGHVKRPMN AFMVWSRGER HKLAQQNPSM QNTEISKQLG CRWKSLTEAE KRPFFQEAQR LKILHREKYP NYKYQPHRRA KVSQRSGILQ PAVASTKLYN LLQWDRNPHA ITYRQDWSRA AHLYSKNQQS FYWQPVDIPT GHLQ


    Database link: Q05738

Properties

Applications

Our Abpromise guarantee covers the use of ab193174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.

Target

  • Function
    Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity). Involved in different aspects of gene regulation including promoter activation or repression (By similarity). Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.
  • Involvement in disease
    Defects in SRY are a cause of gonadal dysgenesis XY female type (GDXY) [MIM:400044]; also known as complete SRY-related 46,XY gonadal dysgenesis or 'XY females' or Swyer syndrome. Patients are found to have a 46,XY karyotype. They suffer rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. In contrast, subjects with 46,XY partial gonadal dysgenesis have ambiguous genitalia, a mix of Muellerian and Wolffian structures, and dysgenic gonads.
    Note=A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, "streak gonads", variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female.
    Defects in SRY are a cause of true hermaphroditism (TRUHER) [MIM:400045]; also known as complete SRY-positive 46,XX gonadal dysgenesis. A true hermaphrodite must have both mature ovarian and mature testicular tissue with histologic evidence of follicles and tubules, respectively. It is a genetically heterogeneous condition. The genotype of most affected individuals is 46,XX, but many have 46,XY or a mosaic of 46,XX/46,XY. True hermaphroditism can be caused also by chromosomal translocation.
  • Sequence similarities
    Belongs to the SRY family.
    Contains 1 HMG box DNA-binding domain.
  • Domain
    DNA binding and bending properties of the HMG domains of human and mouse SRY differ form each other. Human SRY shows more extensive minor groove contacts with DNA and a lower specificity of sequence recognition than mouse SRY.
  • Post-translational
    modifications
    Phosphorylated on serine residues by PKA. Phosphorylation by PKA enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-136 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylated by PARP1. ADP-ribosylation reduces its DNA-binding activity.
  • Cellular localization
    Nucleus speckle. Cytoplasm. Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus. Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH).
  • Information by UniProt
  • Database links
  • Alternative names
    • Essential protein for sex determination in human males antibody
    • Sex determining region on Y antibody
    • Sex determining region protein antibody
    • Sex determining region Y antibody
    • Sex-determining region Y protein antibody
    • sox21b antibody
    • SRXX1 antibody
    • SRXY1 antibody
    • SRY 1 antibody
    • SRY antibody
    • SRY_HUMAN antibody
    • Sry1 antibody
    • Sry3BI antibody
    • SRYGENE antibody
    • TDF antibody
    • TDY antibody
    • Testis determining factor antibody
    • Testis Determining Factor on Y antibody
    • Testis-determining factor antibody
    • Transcription factor Sox-21-B antibody
    see all

References

ab193174 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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