Overview

  • Product nameAnti-SRY antibody [SRY.G12]
    See all SRY primary antibodies
  • Description
    Mouse monoclonal [SRY.G12] to SRY
  • Specificityab22166 recognises specifically SRY in XY testes, but not in XX testes.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Fusion protein (Mouse): SRY

  • Positive control
    • Mouse testes.

Properties

Applications

Our Abpromise guarantee covers the use of ab22166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/5000.
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 24 kDa).

Target

  • FunctionTranscriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity). Involved in different aspects of gene regulation including promoter activation or repression (By similarity). Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.
  • Involvement in diseaseDefects in SRY are a cause of gonadal dysgenesis XY female type (GDXY) [MIM:400044]; also known as complete SRY-related 46,XY gonadal dysgenesis or 'XY females' or Swyer syndrome. Patients are found to have a 46,XY karyotype. They suffer rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. In contrast, subjects with 46,XY partial gonadal dysgenesis have ambiguous genitalia, a mix of Muellerian and Wolffian structures, and dysgenic gonads.
    Note=A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, "streak gonads", variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female.
    Defects in SRY are a cause of true hermaphroditism (TRUHER) [MIM:400045]; also known as complete SRY-positive 46,XX gonadal dysgenesis. A true hermaphrodite must have both mature ovarian and mature testicular tissue with histologic evidence of follicles and tubules, respectively. It is a genetically heterogeneous condition. The genotype of most affected individuals is 46,XX, but many have 46,XY or a mosaic of 46,XX/46,XY. True hermaphroditism can be caused also by chromosomal translocation.
  • Sequence similaritiesBelongs to the SRY family.
    Contains 1 HMG box DNA-binding domain.
  • DomainDNA binding and bending properties of the HMG domains of human and mouse SRY differ form each other. Human SRY shows more extensive minor groove contacts with DNA and a lower specificity of sequence recognition than mouse SRY.
  • Post-translational
    modifications
    Phosphorylated on serine residues by PKA. Phosphorylation by PKA enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-136 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylated by PARP1. ADP-ribosylation reduces its DNA-binding activity.
  • Cellular localizationNucleus speckle. Cytoplasm. Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus. Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH).
  • Information by UniProt
  • Database links
  • Alternative names
    • Essential protein for sex determination in human males antibody
    • Sex determining region on Y antibody
    • Sex determining region protein antibody
    • Sex determining region Y antibody
    • Sex-determining region Y protein antibody
    • sox21b antibody
    • SRXX1 antibody
    • SRXY1 antibody
    • SRY 1 antibody
    • SRY antibody
    • SRY_HUMAN antibody
    • Sry1 antibody
    • Sry3BI antibody
    • SRYGENE antibody
    • TDF antibody
    • TDY antibody
    • Testis determining factor antibody
    • Testis Determining Factor on Y antibody
    • Testis-determining factor antibody
    • Transcription factor Sox-21-B antibody
    see all

Anti-SRY antibody [SRY.G12] images

References for Anti-SRY antibody [SRY.G12] (ab22166)

This product has been referenced in:
  • Bernard P  et al. Wnt signaling in ovarian development inhibits Sf1 activation of Sox9 via the Tesco enhancer. Endocrinology 153:901-12 (2012). Mouse . Read more (PubMed: 22128028) »
  • Sim H  et al. Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads. Endocrinology 152:2883-93 (2011). Read more (PubMed: 21558314) »

See all 4 Publications for this product

Product Wall

Sex-determining region Y protein (Testis-determining factor) Rattus norvegicus (Rat) shows a homology of 86.0% to the immunogen of ab22166 and we would expect a cross reaction of the antibody.

Please also be aware the HMG box transcription fa...

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Thank you for contacting Abcam earlier today about ab22166.

Attached is the Abcam protocols book that contains information on IF and also a host of other useful protocols. The information about the testing discount program is at the end of the...

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Thank you for your reply.


I apologize for any confusion. We do not carry embryonic mouse testis lysates. I can confirm that the WB image is from fetal mouse, so I will update the image caption to avoid further confusion.


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Thank you for your inquiry.

We do sell the positive control mouse testis whole cell lysate as ab29289, but this is something that you would have to purchase.


http://www.abcam.com/Testis-Mouse-Whole-Cell-Lysate-normal-tissue-ab...

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Thank you for your reply.


Mice were immunized with a full length SRY protein and then monoclonal antibodies were derived from that. It is not uncommon to not map the specific sequence of amino acids that is targeted by the monoclonal an...

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Thank you for contacting Abcam regarding ab22166.


The immunogen is the full length protein fused with a proprietary tag. The specific epitope has not been mapped so we do not know where the antibody specifically binds to the protein. Read More

Thank you very much for your interest in ab22166.  This should be a male sex-specific protein. http://www.uniprot.org/uniprot/Q05738 To our knowledge, ab22166 has not been tested in flow cytometry (FC). Therefore, I can offer a discount off a f...

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I was forwarded your enquiry by my colleague, and I have attached the requested certificates of compliance. We don't assign expiration dates to individual products, and our Abpromise guarantee extends for 6 months from purchase as long as the products ...

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Thank you for your enquiry. Ab22166 is only available in the unconjugated form. You could conjugate it yourself (there are kits available) or have another company do it for you. Please note that to our knowledge, this antibody has yet to be tested i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"