Overview

  • Product nameAnti-SS18 antibody
    See all SS18 primary antibodies
  • Description
    Rabbit polyclonal to SS18
  • Specificityab66013 detects endogenous levels of total SS18 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from N terminus of human SS18.

  • Positive control
    • Extracts from HepG2 cells, COLO205 cells and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab66013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionAppears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation.
  • Tissue specificityFairly ubiquitously expressed. Expressed in synovial sarcomas and in other human cell lines. The fusion genes SSXT-SSX1 and SSXT-SSX2 are expressed only in synovial sarcomas.
  • Involvement in diseaseNote=A chromosomal aberration involving SS18 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).
  • Sequence similaritiesBelongs to the SS18 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC116875 antibody
    • Protein SSXT antibody
    • Protein SYT antibody
    • SS18 antibody
    • SSXT antibody
    • SSXT/SSX4v fusion antibody
    • SSXT_HUMAN antibody
    • Synovial sarcoma translocated to X chromosome antibody
    • Synovial sarcoma translocated to X chromosome protein antibody
    • Synovial sarcoma translocation chromosome 18 antibody
    • SYT antibody
    • SYT SSX1 antibody
    • SYT SSX2 antibody
    • SYT/SSX4v fusion protein antibody
    • SYTSSX1 antibody
    • SYTSSX2 antibody
    see all

Anti-SS18 antibody images

  • All lanes : Anti-SS18 antibody (ab66013) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : COLO205 cell extracts
    Lane 3 : Jurkat cell extracts
    Lane 4 : Jurkat cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,30 kDa,80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SS18 antibody (ab66013)

ab66013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"