Overview

  • Product name
  • Description
    Rabbit polyclonal to SSB
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 6 and 242 of Human SSB (Uniprot ID: P05455).

  • Positive control
    • 293T, JC and C2C12 whole cell lysates; A431 cells; Cal27 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
  • Sequence similarities
    Contains 1 HTH La-type RNA-binding domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
    The N-terminus is blocked.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen La antibody
    • La antibody
    • La autoantigen antibody
    • La autoantigen homolog antibody
    • La protein antibody
    • La ribonucleoprotein antibody
    • La ribonucleoprotein domain family member 3 antibody
    • LA_HUMAN antibody
    • LARP3 antibody
    • Lupus La antigen antibody
    • Lupus La protein antibody
    • Lupus La protein homolog antibody
    • MGC118101 antibody
    • MGC93380 antibody
    • mRNA for autoantigen antibody
    • OTTMUSP00000014043 antibody
    • RP23 273G23.1 antibody
    • Sjoegren syndrome type B antigen antibody
    • Sjogren syndrome antigen B (autoantigen La) antibody
    • Sjogren syndrome antigen B antibody
    • SS B antibody
    • SS-B antibody
    • SS-B/La protein antibody
    • SSB antibody
    see all

Images

  • Anti-SSB antibody (ab154998) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 46 kDa
  • All lanes : Anti-SSB antibody (ab154998) at 1/10000 dilution

    Lane 1 : JC whole cell lysate
    Lane 2 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 46 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling SSB with ab154998 at 1/200 dilution. Bottom image shows costaining with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling SSB using ab154998 at 1/100 dilution.

References

ab154998 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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