Overview

  • Product nameAnti-SSB antibody [EPR6570]
    See all SSB primary antibodies
  • Description
    Rabbit monoclonal [EPR6570] to SSB
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Jurkat, K562, HeLa, Ramos, C6, PC12, NIH 3T3 and RAW 264.7 cell lysates; Human colon tissue; Human thyroid adenocarcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionBinds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
    • Sequence similaritiesContains 1 HTH La-type RNA-binding domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
      The N-terminus is blocked.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Autoantigen La antibody
      • La antibody
      • La autoantigen antibody
      • La autoantigen homolog antibody
      • La protein antibody
      • La ribonucleoprotein antibody
      • La ribonucleoprotein domain family member 3 antibody
      • LA_HUMAN antibody
      • LARP3 antibody
      • Lupus La antigen antibody
      • Lupus La protein antibody
      • Lupus La protein homolog antibody
      • MGC118101 antibody
      • MGC93380 antibody
      • mRNA for autoantigen antibody
      • OTTMUSP00000014043 antibody
      • RP23 273G23.1 antibody
      • Sjoegren syndrome type B antigen antibody
      • Sjogren syndrome antigen B (autoantigen La) antibody
      • Sjogren syndrome antigen B antibody
      • SS B antibody
      • SS-B antibody
      • SS-B/La protein antibody
      • SSB antibody
      see all

    Anti-SSB antibody [EPR6570] images

    • All lanes : Anti-SSB antibody [EPR6570] (ab124932) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Ramos cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH 3T3 cell lysate
      Lane 8 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab124932, at 1/250 dilution, staining SSB in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • ab124932, at 1/250 dilution, staining SSB in paraffin-embedded Human thyroid adenocarcinoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-SSB antibody [EPR6570] (ab124932)

    ab124932 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124932.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"