Overview

  • Product nameAnti-SSBP1 antibody
    See all SSBP1 primary antibodies
  • Description
    Rabbit polyclonal to SSBP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 16-65 (HESETTTSLV LERSLNRVHL LGRVGQDPVL RQVEGKNPVT IFSLATNEMW) of Human SSBP1 (NP_003134).

  • Positive control
    • Human Fetal Kidney lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThis protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication.
  • Sequence similaritiesContains 1 SSB domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • Mt SSB antibody
    • Mt-SSB antibody
    • MtSSB antibody
    • PWP1 interacting protein 17 antibody
    • PWP1-interacting protein 17 antibody
    • SENSOR OF SINGLE-STRANDED DNA COMPLEX antibody
    • Single stranded DNA binding protein 1 antibody
    • Single stranded DNA binding protein antibody
    • Single stranded DNA binding protein mitochondrial antibody
    • Single-stranded DNA-binding protein antibody
    • SOSS B1 antibody
    • SOSS COMPLEX, SUBUNIT B1 antibody
    • SSBP 1 antibody
    • SSBP antibody
    • SSBP_HUMAN antibody
    • SSBP1 antibody
    see all

Anti-SSBP1 antibody images

  • All lanes : Anti-SSBP1 antibody (ab116350) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate- 18µg
    Lane 2 : HeLa whole cell lysate- 36µg
    Lane 3 : RPE-1 whole cell lysate- 18µg
    Lane 4 : RPE-1 whole cell lysate- 36µg

    Secondary
    LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 113 kDa (possible non-specific binding).

    Exposure time : 7 minutes

    This image is courtesy of an anonymous abreview.

    Blocking: 10% Milk

    See Abreview

  • Anti-SSBP1 antibody (ab116350) at 1 µg + Human Fetal Kidney lysate at 10 µg

    Predicted band size : 17 kDa
    Gel concentration: 10-20%.

References for Anti-SSBP1 antibody (ab116350)

ab116350 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa, RPE-1)
Specification HeLa, RPE-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"