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Rabbit polyclonal to SSBP1
Predicted to work with:
A synthesized peptide derived from internal sequence of human SSBP1.
Human pancreas tissue; extracts from HeLa cells and HUVEC cells; A549 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
1/50 - 1/100.
1/500 - 1/1000.
This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication.
Contains 1 SSB domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Mt SSB antibody
Western blot - SSBP1 antibody (ab74710)
All lanes :
Anti-SSBP1 antibody (ab74710) at 1/500 dilution
Lane 1 :
Extracts from HeLa cells with no peptide
Lane 2 :
Extracts from HUVEC cells with no peptide
Lane 3 :
Extracts from HUVEC cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size:
Observed band size:
Additional bands at:
26 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Secondary antibody -
anti-rabbit HRP ( ab6721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SSBP1 antibody (ab74710)
Immunohistochemical analysis of paraffin-embedded
human pancreas tissue using ab74710 at a dilution of 1/50. Left-hand panel: no peptide; right-hand panel: with peptide.
Immunocytochemistry/ Immunofluorescence - SSBP1 antibody (ab74710)
Immunofluorescence analysis of A549 cells, using ab74710 at a dilution of 1/500. Left-hand panel: no peptide; right-hand panel: with peptide.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"