Overview

  • Product nameAnti-SSBP1 antibody
    See all SSBP1 primary antibodies
  • Description
    Rabbit polyclonal to SSBP1
  • Tested applicationsSuitable for: WB, IHC-P, Indirect ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from internal sequence of human SSBP1.

  • Positive control
    • Human pancreas tissue; extracts from HeLa cells and HUVEC cells; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100.
Indirect ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionThis protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication.
  • Sequence similaritiesContains 1 SSB domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • Mt SSB antibody
    • Mt-SSB antibody
    • MtSSB antibody
    • PWP1 interacting protein 17 antibody
    • PWP1-interacting protein 17 antibody
    • SENSOR OF SINGLE-STRANDED DNA COMPLEX antibody
    • Single stranded DNA binding protein 1 antibody
    • Single stranded DNA binding protein antibody
    • Single stranded DNA binding protein mitochondrial antibody
    • Single-stranded DNA-binding protein antibody
    • SOSS B1 antibody
    • SOSS COMPLEX, SUBUNIT B1 antibody
    • SSBP 1 antibody
    • SSBP antibody
    • SSBP_HUMAN antibody
    • SSBP1 antibody
    see all

Anti-SSBP1 antibody images

  • All lanes : Anti-SSBP1 antibody (ab74710) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells with no peptide
    Lane 2 : Extracts from HUVEC cells with no peptide
    Lane 3 : Extracts from HUVEC cells with peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 26 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ab74710 at a dilution of 1/50. Left-hand panel: no peptide; right-hand panel: with peptide.
  • Immunofluorescence analysis of A549 cells, using ab74710 at a dilution of 1/500. Left-hand panel: no peptide; right-hand panel: with peptide.

References for Anti-SSBP1 antibody (ab74710)

ab74710 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lymphocytes)
Loading amount 40 µg
Specification Lymphocytes
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"