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F9 teratocarcinoma stem cells (X-irradiated).
Our Abpromise guarantee covers the use of ab16285 in the following tested applications.
|IHC-FoFr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC-FrFl||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.