Overview

  • Product name
    Anti-SSEA1 antibody [MC-480]
  • Description
    Mouse monoclonal [MC-480] to SSEA1
  • Tested applications
    Suitable for: IHC-FoFr, ICC/IF, IP, IHC-P, IHC-FrFl, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    F9 teratocarcinoma stem cells (X-irradiated).

  • Positive control
    • Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.
  • General notes
    Isotype is mouse IgM, kappa light chain. From Solter and Knowles, 1978.

Properties

Applications

Our Abpromise guarantee covers the use of ab16285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
ICC/IF 1/100.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 10 family.
  • Cellular localization
    Golgi apparatus, Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3)-fucosyltransferase 4 antibody
    • Alpha (1,3) fucosyltransferase antibody
    • Alpha (1,3) fucosyltransferase antibody antibody
    • Alpha-(1 antibody
    • CD15 antibody
    • EC=2.4.1. antibody
    • ELAM 1 ligand fucosyltransferase antibody
    • ELAM-1 ligand fucosyltransferase antibody
    • ELAM1 ligand fucosyltransferase antibody
    • Fuc TIV antibody
    • Fuc-TIV antibody
    • Fucosyltransferase 4 antibody
    • Fucosyltransferase IV antibody
    • FucT-IV antibody
    • FUCTIV antibody
    • Fut4 antibody
    • FUT4_HUMAN antibody
    • Galactoside 3 L fucosyltransferase antibody
    • Galactoside 3-L-fucosyltransferase antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA 1 antibody
    • SSEA-1 antibody
    • SSEA1 antibody
    • Stage specific embryonic antigen 1 antibody
    • Stage specific embryonic antigen 1 antibody antibody
    see all

Anti-SSEA1 antibody [MC-480] images

  • The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.
  • Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

    See Abreview

  • IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

References for Anti-SSEA1 antibody [MC-480] (ab16285)

This product has been referenced in:
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
  • Imamura T  et al. Identification of hepta-histidine as a candidate drug for Huntington's disease by in silico-in vitro- in vivo-integrated screens of chemical libraries. Sci Rep 6:33861 (2016). IHC . Read more (PubMed: 27653664) »

See all 24 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Chicken ESC)
Permeabilization
No
Specification
Chicken ESC
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Zebrafish Cultured Cells (embryonic)
Specification
embryonic
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% tritone X 100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Miss. Salma Sana

Verified customer

Submitted Jan 16 2012

Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.

Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some ant...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell (D3))
Specification
embryonic stem cell (D3)
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
live cells
Username

Abcam user community

Verified customer

Submitted Nov 24 2011

Thank you very much for your request. I have put the MSDS in the attachment of this email. The MSDS for ab19857 and ab16285 can also be directly downloaded from their datasheet. The link can be found scrolling down, underneath the "storage buffer". ...

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DISCOUNT CODE: XXXXXXXXX Expiration date: 19-01-2012 I am very pleased to hear you would like to accept our offer and test ab16285 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, plea...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Coronal Brain Sections)
Specification
Coronal Brain Sections
Username

Abcam user community

Verified customer

Submitted May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (mouse ES cell line D3)
Specification
mouse ES cell line D3
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer (gibco) from adherent culture
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample
Username

Abcam user community

Verified customer

Submitted May 03 2011

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (F9 Cells)
Total protein in input
5000 µg
Specification
F9 Cells
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Oct 21 2009

1-10 of 12 Abreviews or Q&A

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