Anti-SSEA3 antibody [MC-631] (Biotin) (ab94857)

Overview

  • Product nameAnti-SSEA3 antibody [MC-631] (Biotin)
    See all SSEA3 primary antibodies
  • Description
    Rat monoclonal [MC-631] to SSEA3 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    4-8 cell stage mouse embryos

  • Positive control
    • NCCIT cells
  • General notesStable for 12 months

Properties

Applications

Our Abpromise guarantee covers the use of ab94857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells. ab35773-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceSSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.
  • Alternative names
    • Stage specific embryonic antigen 3 antibody

Anti-SSEA3 antibody [MC-631] (Biotin) images

  • Image shows cell surface flow cytometric analysis of SSEA3 on NCCIT cells using 0.5 ug of ab94857. Green histogram represents an isotype control; red represents ab94857. A cell surface flow kit and a PE-conjugated streptavidin secondary were used for this test (cells were not fixed for testing).

References for Anti-SSEA3 antibody [MC-631] (Biotin) (ab94857)

ab94857 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"