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Human embryonal carcinoma cell line 2102Ep.
Our Abpromise guarantee covers the use of ab16287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.|
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 15 µg/ml. ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor®588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.
ab16287 staining SSEA4 in human iCell® iPSC Diff Day 0 by Flow Cytometry. Cells were harvested in 1 mM EDTA/1 mM EGTA in PBS (calcium- and magnesium-free) and fixed with acetone. The sample was incubated with the primary antibody (1/200 in 10% FBS) for 2 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Gating Strategy: negative control cells.
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