For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Human embryonal carcinoma cell line 2102Ep.
Our Abpromise guarantee covers the use of ab16287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 15 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab16287 staining SSEA4 in human Fanconi's Anaemia iPS cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/300 in permeabilization buffer) for 1 hour at 4°C. An allophycocyanin-conjugated goat anti-mouse IgG3 polyclonal (1/800) was used as the secondary antibody.
Gating Strategy: Isotype negative control (white).
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor®588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"