Anti-SSH3 antibody (ab76945)
Key features and details
- Rabbit polyclonal to SSH3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SSH3 antibody -
Description
Rabbit polyclonal to SSH3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residues 609 and 659 of human SSH3 (NP_060327.2)
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab76945 was affinity purified using an epitope specific to SSH3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 73 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54961 Human
- Omim: 606780 Human
- SwissProt: Q8TE77 Human
- Unigene: 29173 Human
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Alternative names
- FLJ10928 antibody
- FLJ20515 antibody
- hSSH 3L antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SSH3 with ab76945 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-SSH3 antibody (ab76945) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands. -
ab76945 at 1µg/ml staining SSH3 in HeLa whole cell lysate immunoprecipitated using ab76945 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab76945 has not yet been referenced specifically in any publications.