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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab76945 was affinity purified using an epitope specific to SSH3 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly.
Sequence similarities Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Cytoplasm > cytoskeleton. Nucleus.
Information by UniProt
hSSH 3L antibody
Anti-SSH3 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSH3 antibody (ab76945)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SSH3 with ab76945 at 1/200 (1µg/ml). Detection: DAB.
Western blot - SSH3 antibody (ab76945)
All lanes : Anti-SSH3 antibody (ab76945) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size : 73 kDa Observed band size : 80 kDa ( why is the actual band size different from the predicted?) Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - SSH3 antibody (ab76945)
ab76945 at 1µg/ml staining SSH3 in HeLa whole cell lysate immunoprecipitated using ab76945 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
References for Anti-SSH3 antibody (ab76945)
has not yet been referenced specifically in any publications.
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