For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to SSH3
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 609 and 659 of human SSH3 (NP_060327.2)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab76945 was affinity purified using an epitope specific to SSH3 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly.
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > cytoskeleton. Nucleus.
Information by UniProt
hSSH 3L antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSH3 antibody (ab76945)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SSH3 with ab76945 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-SSH3 antibody (ab76945)
All lanes :
Anti-SSH3 antibody (ab76945) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size:
Observed band size:
80 kDa (
why is the actual band size different from the predicted?
Additional bands at:
51 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-SSH3 antibody (ab76945)
ab76945 at 1µg/ml staining SSH3 in HeLa whole cell lysate immunoprecipitated using ab76945 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"