Synthetic peptide within Human SSH3 aa 582-610 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8TE77
Our Abpromise guarantee covers the use of ab170440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 73 kDa.|
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling SSH3 with ab170440 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labeling SSH3 with ab170440 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of HeLa cells labeling SSH3 with ab170440 at a 1/10 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
ab170440 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"