Overview

  • Product name
  • Description
    Rabbit polyclonal to SSH3BP1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SSH3BP1 aa 483-494 (C terminal).
    Sequence:

    WYEGVCNRVTGL


    Database link: Q8IZP0

  • Positive control
    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab65828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Heat Mediated Antigen Retrieval: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

 

Target

  • Function
    May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.
  • Tissue specificity
    Widely expressed, with highest expression in brain.
  • Involvement in disease
    Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.
  • Sequence similarities
    Belongs to the ABI family.
    Contains 1 SH3 domain.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Domain
    The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
  • Post-translational
    modifications
    In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.
  • Cellular localization
    Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson interactor 1 antibody
    • Abi-1 antibody
    • Abi1 antibody
    • ABI1_HUMAN antibody
    • Abl binding protein 4 antibody
    • Abl interactor 1 antibody
    • Abl interactor protein 1 antibody
    • Abl-binding protein 4 antibody
    • AblBP4 antibody
    • e3B1 antibody
    • Eps8 binding protein antibody
    • Eps8 SH3 domain binding protein antibody
    • Eps8 SH3 domain-binding protein antibody
    • Eps8-binding protein antibody
    • Hssh3bp1 antibody
    • Interactor protein AblBP4 antibody
    • NAP1 antibody
    • Nap1 binding protein antibody
    • Nap1-binding protein antibody
    • Nap1BP antibody
    • Spectrin SH3 domain binding protein 1 antibody
    • Spectrin SH3 domain-binding protein 1 antibody
    • SSH3BP antibody
    see all

Images

  • Immunohistochemical analysis of rat brain tissue labeling SSH3BP1 with ab65828 at 0.5 μg/mL. 

  • Anti-SSH3BP1 antibody (ab65828) at 2 µg/ml + Rat brain lysate

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

ab65828 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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