Overview

  • Product name
  • Description
    Rabbit polyclonal to SSH3BP1
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 325 and 375 of human SSH3BP1 (NP_005461.2).

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 55, 60 kDa (predicted molecular weight: 55 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.
  • Tissue specificity
    Widely expressed, with highest expression in brain.
  • Involvement in disease
    Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.
  • Sequence similarities
    Belongs to the ABI family.
    Contains 1 SH3 domain.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Domain
    The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
  • Post-translational
    modifications
    In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.
  • Cellular localization
    Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson interactor 1 antibody
    • Abi-1 antibody
    • Abi1 antibody
    • ABI1_HUMAN antibody
    • Abl binding protein 4 antibody
    • Abl interactor 1 antibody
    • Abl interactor protein 1 antibody
    • Abl-binding protein 4 antibody
    • AblBP4 antibody
    • e3B1 antibody
    • Eps8 binding protein antibody
    • Eps8 SH3 domain binding protein antibody
    • Eps8 SH3 domain-binding protein antibody
    • Eps8-binding protein antibody
    • Hssh3bp1 antibody
    • Interactor protein AblBP4 antibody
    • NAP1 antibody
    • Nap1 binding protein antibody
    • Nap1-binding protein antibody
    • Nap1BP antibody
    • Spectrin SH3 domain binding protein 1 antibody
    • Spectrin SH3 domain-binding protein 1 antibody
    • SSH3BP antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SSH3BP1 with ab72318 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SSH3BP1 antibody (ab72318) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 55 kDa
    Observed band size : 55,60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human SSH3BP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab72317 used for IP at 3 µg/mg lysate. Lane 2: SSH3BP1 immunoprecipitated with an other antibody. Lane 3: ab72318 at 3 µg/mg lysate used for IP of SSH3BP1, which recognize downstream epitopes. Lane 4: control Ig IP For blotting immunoprecipitated SSH3BP1, ab72318 was used at 1 µg/ml.
  • Detection of SSH3BP1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72318 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References

ab72318 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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