Overview

  • Product nameAnti-SSRP1 antibody
    See all SSRP1 primary antibodies
  • Description
    Rabbit polyclonal to SSRP1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 75 and 308 of Human SSRP1 (NP_003137)

  • Positive control
    • Molt-4 whole cell lysate and Cal27 xenograft tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.
IHC-P 1/100 - 1/250.

Target

  • FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
  • Sequence similaritiesBelongs to the SSRP1 family.
    Contains 1 HMG box DNA-binding domain.
  • DomainThe HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
  • Post-translational
    modifications
    Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
    Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
    Sumoylated.
  • Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
    • Cisplatin DNA SSRP antibody
    • Facilitates chromatin remodeling 80 kDa subunit antibody
    • Facilitates chromatin transcription complex 80 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SSRP1 antibody
    • FACT 80 kDa subunit antibody
    • FACT complex subunit SSRP1 antibody
    • FACT80 antibody
    • FACTp80 antibody
    • hSSRP1 antibody
    • Recombination signal sequence recognition protein 1 antibody
    • Recombination signal sequence recognition protein antibody
    • SSRP 1 antibody
    • SSRP1 antibody
    • SSRP1_HUMAN antibody
    • Structure specific recognition protein 1 antibody
    • Structure-specific recognition protein 1 antibody
    • T160 antibody
    see all

Anti-SSRP1 antibody images

  • Anti-SSRP1 antibody (ab96704) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 81 kDa
  • ab96704, at a 1/100 dilution, staining human SSRP1 in Cal27 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References for Anti-SSRP1 antibody (ab96704)

ab96704 has not yet been referenced specifically in any publications.

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