Overview

  • Product nameAnti-SSSCA1 antibody [2F5]
    See all SSSCA1 primary antibodies
  • Description
    Mouse monoclonal [2F5] to SSSCA1
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human SSSCA1 produced in HEK293T cells (NP_006387).

  • Positive control
    • HEK293T cells and cell lysate transfected with pCMV6-ENTRY SSSCA1 cDNA; HeLa and Jurkat cells; COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab117911 is purified from Mouse ascites fluid by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number2F5
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 21 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionMight play a role in mitosis. Antigenic molecule. Could be a centromere-associated protein. May induce anti-centromere antibodies.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500016H19Rik antibody
    • Autoantigen p27 antibody
    • C184L antibody
    • centromeric autoantigen (27kD) antibody
    • OTTHUMP00000232769 antibody
    • OTTHUMP00000233069 antibody
    • OTTHUMP00000233071 antibody
    • p27 antibody
    • Sjoegren syndrome/scleroderma autoantigen 1 antibody
    • Sjogren syndrome/scleroderma autoantigen 1 antibody
    • Sjogren's syndrome/scleroderma autoantigen 1 homolog antibody
    • SSA27_HUMAN antibody
    • SSSCA1 antibody
    see all

Anti-SSSCA1 antibody [2F5] images

  • ab117911 at 1/100 dilution staining SSSCA1 in COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1 by Immunofluorescence.
  • HEK293T cells transfected with either pCMV6-ENTRY SSSCA1 overexpress plasmid(Red) or empty vector control plasmid (Blue), immunostained using ab117911 at 1/100 dilution and then analyzed by flow cytometry.
  • ab117911 at 1/100 dilution staining SSSCA1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab117911 at 1/100 dilution staining SSSCA1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • All lanes : Anti-SSSCA1 antibody [2F5] (ab117911) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SSSCA1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 21 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200609)

References for Anti-SSSCA1 antibody [2F5] (ab117911)

ab117911 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"