Anti-SSX1 antibody [1E10] (ab119036)

Overview

  • Product nameAnti-SSX1 antibody [1E10]
    See all SSX1 primary antibodies
  • Description
    Mouse monoclonal [1E10] to SSX1
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human SSX1 produced in E.coli (NP_005626).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY SSX1 cDNA; COS7 cells transiently transfected with pCMV6-ENTRY SSX1; HEK293T cells transfected with a SSX1 overexpress plasmid; Hela cells; Jurkat cells
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionCould act as a modulator of transcription.
  • Tissue specificityExpressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines.
  • Involvement in diseaseNote=A chromosomal aberration involving SSX1 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).
  • Sequence similaritiesBelongs to the SSX family.
    Contains 1 KRAB-related domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 5.1 antibody
    • Cancer/testis antigen family 5 member 1 antibody
    • CT5.1 antibody
    • MGC150425 antibody
    • MGC5162 antibody
    • Protein SSX1 antibody
    • sarcoma, synovial, X chromosome related 1 antibody
    • SSRC antibody
    • SSX1 antibody
    • SSX1_HUMAN antibody
    • Synovial sarcoma antibody
    • Synovial sarcoma, X breakpoint 1 antibody
    • X breakpoint 1 antibody
    see all

Anti-SSX1 antibody [1E10] images

  • All lanes : Anti-SSX1 antibody [1E10] (ab119036) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SSX1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 22 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201600)
  • ab119036, at 1/100 dilution, staining SSX1 in COS7 cells transiently transfected with pCMV6-ENTRY SSX1 by Immunofluorescence.
  • HEK293T cells transfected with either a SSX1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119036 at 1/100 dilution and then analyzed by flow cytometry.
  • Flow cytometric analysis of SSX1 in Hela cells, using ab119036 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of SSX1 in Jurkat cells using ab119036 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-SSX1 antibody [1E10] (ab119036)

ab119036 has not yet been referenced specifically in any publications.

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