Overview

  • Product nameAnti-SSX2 antibody [4D10]
    See all SSX2 primary antibodies
  • Description
    Mouse monoclonal [4D10] to SSX2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Protein expressed in 293T cell transfected with Human SSX2 expression vector.

  • Positive control
    • WB: SSX2 transfected HEK293T cell lysate ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY SSX2 IHC-P: Human adenocarcinoma of breast, colon, adenocarcinoma of colon, kidney, ovary, pancreas, carcinoma of thyroid, endometrium, prostate, and carcinoma of bladder tissues
  • General notesDilute in PBS (pH 7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab117972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 22 kDa.
IHC-P 1/50.
ICC/IF 1/100.

Target

  • FunctionCould act as a modulator of transcription.
  • Tissue specificityExpressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines.
  • Involvement in diseaseNote=A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).
  • Sequence similaritiesBelongs to the SSX family.
    Contains 1 KRAB-related domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 5.2 antibody
    • Cancer/testis antigen family 5, member 2a antibody
    • Cancer/testis antigen family 5, member 2b antibody
    • CT5.2 antibody
    • CT5.2A antibody
    • CT5.2b antibody
    • HD21 antibody
    • hom mel 40 antibody
    • HOM-MEL-40 antibody
    • MGC119055 antibody
    • MGC15364 antibody
    • MGC3884 antibody
    • Protein SSX2 antibody
    • RP11-552J9.2 antibody
    • sarcoma, synovial, X-chromosome related 2 antibody
    • SSx antibody
    • SSX2 antibody
    • SSX2-SYT fusion gene, included antibody
    • SSX2_HUMAN antibody
    • SSX2B antibody
    • Synovial sarcoma antibody
    • synovial sarcoma X breakpoint 2 antibody
    • Synovial sarcoma, X breakpoint 2B antibody
    • Tumor antigen HOM-MEL-40 antibody
    • X breakpoint 2 antibody
    see all

Anti-SSX2 antibody [4D10] images

  • All lanes : Anti-SSX2 antibody [4D10] (ab117972) at 1/2000 dilution

    Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control
    Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY SSX2

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 22 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201214)
  • Immunohistochemical staining of paraffin embedded Human Adenocarcinoma of breast tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human colon, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human Adenocarcinoma of colon tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human kidney tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human ovary tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human prostate tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human Carcinoma of bladder tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human Carcinoma of thyroid tissue, using ab117972 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin embedded Human endometrium tissue, using ab117972 at a dilution of 1/50.
  • Immunofluorescent staining of COS7 cells transiently transfected by SSX2, using ab117972 at a dilution of 1/100.

References for Anti-SSX2 antibody [4D10] (ab117972)

ab117972 has not yet been referenced specifically in any publications.

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